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Nucleic acid nrhiav kom tau fluorescence quantitative Ct tus nqi - ib qho kev hlub-ntxhais parameter

Nucleic acid nrhiav kom tau fluorescence quantitative Ct tus nqi - ib qho kev hlub-ntxhais parameter

Qhov "Tshiab Coronary Virus Pneumonia Diagnosis and Treatment Program (Trial Ninth Edition)" siv cov nucleic acid nrhiav Ct tus nqi ≥35 ua ib lub hauv paus tseem ceeb rau kev tso tawm ntawm kev tswj hwm kev cais tawm lossis tso tawm.Yog li, Ct tus nqi ntawm cov khoom siv tshuaj ntsuam xyuas nucleic acid sawv cev li cas?Puas yog Ct qhov tseem ceeb ntawm cov khoom siv sib txawv?Puas yog tus nqi Ct qis dua, qhov ua tau zoo ntawm cov khoom siv?
Tus nqi Ct (Threshold Cycle, Ct) yog tus naj npawb ntawm PCR cycles thaum qhov kev siv ntawm lub sijhawm tiag tiag fluorescence quantitative fluorescence signal tshaj qhov pib.Nucleic acid kuaj cov khoom siv coj cov yas tshiab ua piv txwv.Rau tib cov tshuaj tiv thaiv ntawm tib cov khoom siv txhawm rau txhawm rau kuaj ob tus kab mob uas muaj tus kab mob, A thiab B, qhov loj ntawm Ct tus nqi sawv cev, rau qee qhov, tus naj npawb ntawm cov ntawv luam ntawm cov kab mob kis, uas yog, cov kab mob kis.Qhov qis dua Ct ntawm tus qauv B, qhov siab dua qhov kis kab mob.Tus nqi Ct yog inversely proportional rau tus kab mob load thiab nws kis.Qhov zoo thiab qhov tsis zoo ntawm cov khoom siv fluorescence sib txawv ntawm lub sijhawm tiag tiag tsis tuaj yeem txiav txim los ntawm qhov loj ntawm Ct tus nqi, vim tias Ct qhov tseem ceeb ntawm cov khoom siv sib txawv tsis sib piv.Kev ntsuas kev ua tau zoo ntawm cov khoom siv yuav tsum tau ntsuas los ntawm cov yam ntxwv ntawm qhov rhiab heev (qhov tsawg kawg ntawm kev kuaj pom), qhov tshwj xeeb, qhov tseeb, kev ruaj ntseg, thiab daim ntawv thov kev kuaj mob.Txhua lub chaw tsim khoom reagent muaj nws tus kheej kaum yim hom kev ua yeeb yam, thiab lawv muaj lawv tus kheej tej yam yees siv powers hauv daim ntawv thov, yog li kuv yuav tsis mus rau hauv cov ntsiab lus ntawm no.
Rau cov pa hluav taws xob hauv lub sijhawm tiag tiag ntawm PCR (QPCR) cov cuab yeej / cov tshuab, kev nrhiav tau ntawm Ct qhov tseem ceeb thiab lawv cov kev siv dav dav hauv kev siv tshuaj tua kab mob yog paub rau txhua tus.Nrog rau kev txhim kho kev lag luam thiab kev nce qib ntawm kev siv thev naus laus zis, QPCR cov cuab yeej siv thiab cov tshuaj reagents tau ntsib cov hauv kev thiab cov teeb meem.Yiou Xav Tank (2021 Kev Tshawb Fawb Tshaj Tawm ntawm Tuam Tshoj Kev Tshawb Fawb Kev Lag Luam Kev Lag Luam: Technology) tau txheeb xyuas tias feem ntau cov tuam txhab hauv kuv lub teb chaws QPCR kev lag luam tab tom ntsib teeb meem tshwm sim raws li kev siv tshuab.Hauv kev teb rau cov teeb meem no, cov kev daws teeb meem feem ntau yog muab faib ua ob hom: kev txhim kho thev naus laus zis thiab siv cov thev naus laus zis tshiab.
POCT rau QPCR molecular diagnosis yog ib qho ntawm kev txhim kho cov lus qhia ntawm kev lag luam, thiab kev txhim kho kev ua tau zoo yog qhov tseem ceeb rau kev ua kom nrawm ntawm kev siv tshuab.Rau cov khoom siv POCT nrog ib qho qauv dhau los ntawm QPCR, yuav ua li cas txhawm rau txhim kho Ct tus nqi ntawm qhov tseem ceeb ntawm ib qho kev tshwm sim hauv R & D?Cov khoom siv tib yam, tib cov tshuaj tiv thaiv kab mob, thiab tib lub template concentration, theoretically qhov loj dua tus nqi Ct ntawm ib qho tshuaj tiv thaiv, qhov qis dua ntawm cov enzyme hauv cov tshuaj tiv thaiv.
Nyob rau hauv tus txheej txheem ntawm kev txhim kho cov kev ua tau zoo ntawm cov tshuaj tiv thaiv enzyme thiab optimizing tus nqi Ct, ib tug raug 40 PCR voj voog fluorescence quantitative amplification nkhaus tuaj yeem txheeb xyuas ua ntej.Lub amplification nkhaus nyob rau hauv daim duab hauv qab no yog muab faib ua fluorescence keeb kwm yav dhau theem, exponential amplification theem, linear theem, thiab toj siab theem.Txhawm rau kom ua tiav qhov qis dua fluorescence keeb kwm yav dhau los, kom deb li deb tau kom paub qhov txawv ntawm qhov fluorescence tus nqi ntawm kev nthuav dav thaum ntxov, nws yog ib qho tsim nyog yuav tsum tau sim los ntawm cov tshuaj tiv thaiv cov khoom siv hluav taws xob, kev tshuaj ntsuam xyuas primer, thiab cov tshuaj tiv thaiv tsis zoo.Lub sijhawm exponential amplification yog cov tshuaj tiv thaiv ncaj qha tshaj plaws ntawm kev ua enzyme, thiab nws tseem yog cov pov thawj ntawm kev sib koom tes zoo tshaj plaws ntawm POCT cov cuab yeej, cov tshuaj tiv thaiv thiab cov tshuaj reagents.Lub sijhawm no, cov cuab yeej tswj qhov kub thiab txias, kho qhov muag teeb pom kev zoo thiab kev tsom xam, cov khoom siv biocompatibility, thiab kev ua haujlwm ntawm cov tshuaj reagents yuav tsum tau kho thiab kuaj txhua yam.Thaum kawg, tag nrho cov amplification tiav, thiab cov txiaj ntsig yuav tsum tau nthuav tawm los ntawm cov ntaub ntawv nruj heev kom tau txais tus nqi tsim nyog Ct.
CT kab
Ib kauj ruam me me hauv kev ua kom zoo ntawm tus nqi Ct yog qhov suav tsis txheeb ntawm cov neeg ua haujlwm R & D.Nyob rau hauv kev ntxhov siab thiab kev zoo siab ntawm nruab hnub thiab hmo ntuj, txhua zaus "cov roob thiab cov dej ntws tsis pom qhov twg, cov willows tsaus thiab cov paj ci ntsa iab thiab muaj lwm lub zos", uas ua rau peb lub siab tawv thiab mob siab rau mus tom ntej. dua.


Post lub sij hawm: Sep-29-2022